Neutralizing antibody and protective immunity to SARS coronavirus infection of mice induced by a soluble recombinant polypeptide containing an N-terminal segment of the spike glycoprotein.
Identifieur interne : 004743 ( Main/Exploration ); précédent : 004742; suivant : 004744Neutralizing antibody and protective immunity to SARS coronavirus infection of mice induced by a soluble recombinant polypeptide containing an N-terminal segment of the spike glycoprotein.
Auteurs : Himani Bisht [États-Unis] ; Anjeanette Roberts ; Leatrice Vogel ; Kanta Subbarao ; Bernard MossSource :
- Virology [ 0042-6822 ] ; 2005.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antiviraux (sang), Baculoviridae, Cellules HeLa, Cellules cultivées, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (), Glycoprotéines membranaires (administration et posologie), Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (immunologie), Humains, Immunisation, Peptides (), Peptides (administration et posologie), Peptides (génétique), Peptides (immunologie), Protéines de l'enveloppe virale (), Protéines de l'enveloppe virale (administration et posologie), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (immunologie), Protéines recombinantes (administration et posologie), Protéines recombinantes (génétique), Protéines recombinantes (immunologie), Solubilité, Souris, Spodoptera, Syndrome respiratoire aigu sévère (), Syndrome respiratoire aigu sévère (immunologie), Tests de neutralisation, Vaccins antiviraux (administration et posologie), Vaccins antiviraux (génétique), Vaccins antiviraux (immunologie), Virus du SRAS (immunologie), Virus du SRAS (pathogénicité).
- MESH :
- administration et posologie : Glycoprotéines membranaires, Peptides, Protéines de l'enveloppe virale, Protéines recombinantes, Vaccins antiviraux.
- génétique : Glycoprotéines membranaires, Peptides, Protéines de l'enveloppe virale, Protéines recombinantes, Vaccins antiviraux.
- immunologie : Glycoprotéines membranaires, Peptides, Protéines de l'enveloppe virale, Protéines recombinantes, Syndrome respiratoire aigu sévère, Vaccins antiviraux, Virus du SRAS.
- pathogénicité : Virus du SRAS.
- sang : Anticorps antiviraux.
- Animaux, Baculoviridae, Cellules HeLa, Cellules cultivées, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires, Humains, Immunisation, Peptides, Protéines de l'enveloppe virale, Solubilité, Souris, Spodoptera, Syndrome respiratoire aigu sévère, Tests de neutralisation.
English descriptors
- KwdEn :
- Animals, Antibodies, Viral (blood), Baculoviridae, Cells, Cultured, HeLa Cells, Humans, Immunization, Membrane Glycoproteins (administration & dosage), Membrane Glycoproteins (chemistry), Membrane Glycoproteins (genetics), Membrane Glycoproteins (immunology), Mice, Neutralization Tests, Peptides (administration & dosage), Peptides (chemistry), Peptides (genetics), Peptides (immunology), Recombinant Proteins (administration & dosage), Recombinant Proteins (genetics), Recombinant Proteins (immunology), SARS Virus (immunology), SARS Virus (pathogenicity), Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (prevention & control), Solubility, Spike Glycoprotein, Coronavirus, Spodoptera, Viral Envelope Proteins (administration & dosage), Viral Envelope Proteins (chemistry), Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology), Viral Vaccines (administration & dosage), Viral Vaccines (genetics), Viral Vaccines (immunology).
- MESH :
- chemical , administration & dosage : Membrane Glycoproteins, Peptides, Recombinant Proteins, Viral Envelope Proteins, Viral Vaccines.
- chemical , blood : Antibodies, Viral.
- chemical , chemistry : Membrane Glycoproteins, Peptides, Viral Envelope Proteins.
- chemical , genetics : Membrane Glycoproteins, Peptides, Recombinant Proteins, Viral Envelope Proteins, Viral Vaccines.
- chemical , immunology : Membrane Glycoproteins, Peptides, Recombinant Proteins, Viral Envelope Proteins, Viral Vaccines.
- immunology : SARS Virus, Severe Acute Respiratory Syndrome.
- pathogenicity : SARS Virus.
- prevention & control : Severe Acute Respiratory Syndrome.
- Animals, Baculoviridae, Cells, Cultured, HeLa Cells, Humans, Immunization, Mice, Neutralization Tests, Solubility, Spike Glycoprotein, Coronavirus, Spodoptera.
Abstract
A secreted, glycosylated polypeptide containing amino acids 14 to 762 of the SARS coronavirus (SARS-CoV) spike protein and a polyhistidine tag was expressed in recombinant baculovirus-infected insect cells. Mice received the affinity-purified protein with either a saponin (QS21) or a Ribi (MPL + TDM) adjuvant subcutaneously and were challenged intranasally with SARS-CoV. Both regimens induced binding and neutralizing antibodies and protection against SARS-CoV intranasal infection. However, the best results were obtained with QS21 and protein, which provided the highest antibody as well as complete protection of the upper and lower respiratory tract.
DOI: 10.1016/j.virol.2005.01.042
PubMed: 15780866
Affiliations:
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Le document en format XML
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Coronavirus</term><term>Spodoptera</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Baculoviridae</term><term>Cellules HeLa</term><term>Cellules cultivées</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires</term><term>Humains</term><term>Immunisation</term><term>Peptides</term><term>Protéines de l'enveloppe virale</term><term>Solubilité</term><term>Souris</term><term>Spodoptera</term><term>Syndrome respiratoire aigu sévère</term><term>Tests de neutralisation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A secreted, glycosylated polypeptide containing amino acids 14 to 762 of the SARS coronavirus (SARS-CoV) spike protein and a polyhistidine tag was expressed in recombinant baculovirus-infected insect cells. Mice received the affinity-purified protein with either a saponin (QS21) or a Ribi (MPL + TDM) adjuvant subcutaneously and were challenged intranasally with SARS-CoV. Both regimens induced binding and neutralizing antibodies and protection against SARS-CoV intranasal infection. However, the best results were obtained with QS21 and protein, which provided the highest antibody as well as complete protection of the upper and lower respiratory tract.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Maryland</li></region></list><tree><noCountry><name sortKey="Moss, Bernard" sort="Moss, Bernard" uniqKey="Moss B" first="Bernard" last="Moss">Bernard Moss</name><name sortKey="Roberts, Anjeanette" sort="Roberts, Anjeanette" uniqKey="Roberts A" first="Anjeanette" last="Roberts">Anjeanette Roberts</name><name sortKey="Subbarao, Kanta" sort="Subbarao, Kanta" uniqKey="Subbarao K" first="Kanta" last="Subbarao">Kanta Subbarao</name><name sortKey="Vogel, Leatrice" sort="Vogel, Leatrice" uniqKey="Vogel L" first="Leatrice" last="Vogel">Leatrice Vogel</name></noCountry><country name="États-Unis"><region name="Maryland"><name sortKey="Bisht, Himani" sort="Bisht, Himani" uniqKey="Bisht H" first="Himani" last="Bisht">Himani Bisht</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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